Rita Zgheib, Issam Hasni, Babacar Mbaye, Hussein Anani, Gabriel Haddad, Nicholas Armstrong, Céline Chartier, Aurélia Caputo, Didier Raoult, Florence Fenollar & Pierre-Edouard Fournier
04 January 2022
Current Microbiology
DOI : 10.1007/s00284-021-02714-3
ABSTRACT
Strain Marseille-P9829 was isolated from a bone sample collected from an open right fibula fracture from a 46-years old patient. Strain Marseille-P9829 (= CSUR P9829 = DSM 110695) was a Gram-negative, non-spore-forming and non-motile bacterium. This strain had a positive catalase activity but was oxidase-negative. The major fatty acids methyl esters were hexadecanoic acid (45.6%) and 9-hexadecenoic acid (28.4%). Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry analysis suggested that this strain belongs to the species Buttiauxella gaviniae. Since there were few reports of clinical infections with this species in humans, whole genome sequencing was performed and a polyphasic taxono-genomic approach was followed in order to verify the classification of strain Marseille-P9829. The 16S rRNA gene sequence BLAST against the NCBI database yielded the highest similarity of 99.8% with Buttiauxella agrestis, suggesting that strain Marseille-P9829 belongs to this species. However, genomic comparison by digital DNA–DNA hybridization showed that values between strain Marseille-P9829 and other validly published Buttiauxella species were all lower than 70%. Furthermore, all average nucleotide identities were lower than 95–96%. Therefore, these results confirmed that strain Marseille-P9829 belonged to a new Buttiauxellaspecies for which we propose the name Buttiauxella massiliensis sp. nov., with strain Marseille-P9829 as type strain.